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Demet Tademir Ayegül Karaküük‐yidoan Mustafa Ulali Tuba Takin‐Tok Emne Eln Oru‐Emre Hasan Bayram 《Chirality》2015,27(2):177-188
A series of new chiral thiosemicarbazones derived from homochiral amines in both enantiomeric forms were synthesized and evaluated for their in vitro antiproliferative activity against A549 (human alveolar adenocarcinoma), MCF‐7 (human breast adenocarcinoma), HeLa (human cervical adenocarcinoma), and HGC‐27 (human stomach carcinoma) cell lines. Some of compounds showed inhibitory activities on the growth of cancer cell lines. Especially, compound 17b exhibited the most potent activity (IC50 4.6 μM) against HGC‐27 as compared with the reference compound, sindaxel (IC50 10.3 μM), and could be used as a lead compound to search new chiral thiosemicarbazone derivatives as antiproliferative agents. Chirality 27:177–188, 2015. © 2014 Wiley Periodicals, Inc. 相似文献
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城市化背景下人类与自然环境的矛盾呈现出多尺度、层级化特征,而传统生态网络的构建方式较少考虑不同尺度下生态要素的关系,无法从区域落实到中心城区,难以形成系统性的解决方案。研究在综合梳理各尺度生态网络构建方法的基础上,以长沙市为例,基于形态学空间格局分析(Morphological Spatial Pattern Analysis,MSPA)、景观连通性原理和生态斑块重要性评价识别生态源地,并通过多层级生态阻力面的确定,综合运用最小费用路径(Least-cost path method,LCP)、电路理论、层级传导理论、尺度嵌套等方法对市域、都市区、中心城区的生态网络进行了协同构建和层级优化,最后基于不同尺度生态网络的特点应用并落实到多层级的国土空间规划体系中。研究结果表明:(1)长沙市域生态网络和都市区生态网络具有较好的层级嵌套特征;共识别两尺度生态叠合源地14个、生态叠合廊道15条,主要通过中心城区内的湘江、浏阳河和捞刀河部分河段与外围生态绿圈相衔接,形成"外环内楔"的空间格局。(2)确定市域重要廊道、市域潜在廊道、生态叠合廊道、都市区重要廊道、都市区潜在廊道的核心保护面积共501.14 km2,并提取位于生态廊道核心保护区范围内的生态夹点和生态障碍点,以进一步落实生态保护修复策略。(3)得到具有重要生态连通功能的中心城区生态绿道长度441.2 km,生态修复单元56个,并结合生态阻力值划分为5级进行针对性修复。(4)基于不同尺度生态网络的衔接、嵌套,最终构建"市域总体生态安全格局-都市区城市生态空间发展格局-以城市绿道为基础的中心城区生态修复单元",并与不同层级的国土空间规划体系相对应。研究结果将为以大城市为中心的跨尺度生态系统修复和生态安全格局构建提供科学参考。 相似文献
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The adsorption of chiral Gly‐Pro dipeptide on Cu(110) has been characterized by combining in situ polarization modulation infrared reflection absorption spectroscopy (PM‐RAIRS) and X‐ray photoelectron spectroscopy (XPS). The chemical state of the dipeptide, and its anchoring points and adsorption geometry, were determined at various coverage values. Gly‐Pro molecules are present on Cu(110) in their anionic form (NH2/COO−) and adsorb under a 3‐point binding via both oxygen atoms of the carboxylate group and via the nitrogen atom of the amine group. Low‐energy electron diffraction (LEED) and scanning tunneling microscopy (STM) have shown the presence of an extended 2D chiral array, sustained via intermolecular H‐bonds interactions. Furthermore, due to the particular shape of the molecule, only one homochiral domain is formed, creating thus a truly chiral surface. Chirality 27:411–416, 2015. © 2015 Wiley Periodicals, Inc. 相似文献
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We present a methodology of lipid nanotubes (LNT) and nanodisks technologies optimized in our laboratory for structural studies of membrane‐associated proteins at close to physiological conditions. The application of these lipid nanotechnologies for structure determination by cryo‐electron microscopy (cryo‐EM) is fundamental for understanding and modulating their function. The LNTs in our studies are single bilayer galactosylceramide based nanotubes of ~20 nm inner diameter and a few microns in length, that self‐assemble in aqueous solutions. The lipid nanodisks (NDs) are self‐assembled discoid lipid bilayers of ~10 nm diameter, which are stabilized in aqueous solutions by a belt of amphipathic helical scaffold proteins. By combining LNT and ND technologies, we can examine structurally how the membrane curvature and lipid composition modulates the function of the membrane‐associated proteins. As proof of principle, we have engineered these lipid nanotechnologies to mimic the activated platelet's phosphtaidylserine rich membrane and have successfully assembled functional membrane‐bound coagulation factor VIII in vitro for structure determination by cryo‐EM. The macromolecular organization of the proteins bound to ND and LNT are further defined by fitting the known atomic structures within the calculated three‐dimensional maps. The combination of LNT and ND technologies offers a means to control the design and assembly of a wide range of functional membrane‐associated proteins and complexes for structural studies by cryo‐EM. The presented results confirm the suitability of the developed methodology for studying the functional structure of membrane‐associated proteins, such as the coagulation factors, at a close to physiological environment. Proteins 2014; 82:2902–2909. © 2014 Wiley Periodicals, Inc. 相似文献
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KEVIN D. CHASE DAVE KELLY ANDREW M. LIEBHOLD MARTIN K.‐F. BADER ECKEHARD G. BROCKERHOFF 《Ecological Entomology》2017,42(2):173-183
1. Dispersal and host detection are behaviours promoting the spread of invading populations in a landscape matrix. In fragmented landscapes, the spatial arrangement of habitat structure affects the dispersal success of organisms. 2. The aim of the present study was to determine the long distance dispersal capabilities of two non‐native pine bark beetles (Hylurgus ligniperda and Hylastes ater) in a modified and fragmented landscape with non‐native pine trees. The role of pine density in relation to the abundance of dispersing beetles was also investigated. 3. This study took place in the Southern Alps, New Zealand. A network of insect panel traps was installed in remote valleys at known distances from pine resources (plantations or windbreaks). Beetle abundance was compared with spatially weighted estimates of nearby pine plantations and pine windbreaks. 4. Both beetles were found ≥25 km from the nearest host patch, indicating strong dispersal and host detection capabilities. Small pine patches appear to serve as stepping stones, promoting spread through the landscape. Hylurgus ligniperda (F.) abundance had a strong inverse association with pine plantations and windbreaks, whereas H. ater abundance was not correlated with distance to pine plantations but positively correlated with distance to pine windbreaks, probably reflecting differences in biology and niche preferences. Host availability and dispersed beetle abundance are the proposed limiting factors impeding the spread of these beetles. 5. These mechanistic insights into the spread and persistence of H. ater and H. ligniperda in a fragmented landscape provide ecologists and land managers with a better understanding of factors leading to successful invasion events, particularly in relation to the importance of long‐distance dispersal ability and the distribution and size of host patches. 相似文献
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Development and evaluation of Trichoderma asperellum preparation for control of sheath blight of rice (Oryza sativa L.) 总被引:1,自引:0,他引:1
Li-Hua Chen Jie Zhang Sha-Sha Wang Qi-Song Miao Xin-Yu Mao 《Biocontrol Science and Technology》2015,25(3):316-328
Sheath blight, which is caused by Rhizoctonia solani, is a disease that majorly impacts rice production. A biocontrol agent used for control rice sheath blight must be sprayed on the stem at specific times during rice growth, a process that is labour-intensive and renders the antagonist vulnerable to environmental factors. In this study, Trichoderma asperellum T12 was used to produce preparation by solid-state fermentation using a surface-response method. Rice hull was selected as a carrier based on its ability to sustain the T12 floating in the water and protect T12 from ultraviolet irradiation. The production of a T12-based preparation required 32% wheat bran, 7% inoculum, 2.3 g kg?1 (NH4)2SO4 and 65% water content, with fermentation at 27.5°C for 30 days and agitation every six days. The preparation demonstrated 90% biocontrol efficacy and significantly (P > 0.05) increased the seed-set rate and 1000-grain weight as compared with the pathogen treatment. The population of Trichoderma on the surface of rice leaf sheath in the treatment applied with T12 preparation increased from 232 cfu (colony forming units) g?1 fw (fresh weight) to 436 cfu g?1 fw during rice growth stage, which was significantly (P > 0.05) higher than pathogen treatment. The population of R. solani on the leaf sheath increased from 41 cfu g?1 fw to 271 cfu g?1 fw in the pathogen treatment, while remained stable (P > 0.05) at level of 10–23 cfu g?1 fw in T12 preparation applied treatment. Biocontrol of sheath blight by the addition of the preparation to the soil is effective and decreases the costs of agro-industrial waste disposal. 相似文献